Semen preparation for Assisted Reproductive Technology (ART) involves the separation of the motile sperm form non-motile, dead sperm, cellular debris and seminal fluid. The aim of this study is to compare the motile sperm recovery rate and
motility
index of different sperm preparation method ( swim-up method, 80% isotonic continuous percoll method, two-layer discontinuous percoll method, mini-percoll method). In Mini-Percoll method, pellets from patients were suspended in 0.3ml of medium
and
layerd on a discontinuous percoll gradient consisting of 0.3ml each of 50, 70, 95% isotonic percoll. All semen samples are divided into normal and subnormal sperm group (oligo-, astheno-, oligoasthenozoospermia). Especially, we evaluated the
effect
of
mini-percoll method in subnormal sperm group.
In normal sperm group, mini-percoll method and two layer discontinuous percoll method (40$/80%) allowed increasing of motile sperm recovery rate. But motility index was higher in swim-up method than the other methods. In subnormal sperm group,
mini-percoll method has advantages as compare with the other methods in motile sperm recovery rate and motility index.
These results suggest that modified mini-percoll method could be certainly a valuable tool in some cases of severe male factor sperm.
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